Isolation and identification of bacteria causing otitis media, and testing their susceptibility to certain types of antibiotics
DOI:
https://doi.org/10.65405/0xtbmf46Keywords:
Otitis media, Streptococcus pneumoniae , Pseudomonas aeruginosa, ,Staphylococcus aureus, antibioticsAbstract
Otitis media (OM) is a common infection, particularly among children, and may lead to complications such as hearing impairment or permanent hearing loss. Identification of the bacterial pathogens associated with OM is essential for guiding effective antimicrobial therapy. This study aimed to isolate and identify the bacterial pathogens causing OM and to determine their antimicrobial susceptibility patterns.
The study was conducted from January to June 2024. A total of 40 ear swab specimens were collected from patients diagnosed with OM who attended the outpatient clinics of Sorman Teaching Hospital and Al-Allalaqa Polyclinic. The specimens were cultured, and the bacterial isolates were identified using standard microbiological methods. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disk diffusion method.
Streptococcus pneumoniae was the most frequently isolated pathogen (43%), followed by Pseudomonas aeruginosa (36%) and Staphylococcus aureus (21%). Among the antibiotics tested, levofloxacin and ceftriaxone exhibited the highest susceptibility rates (100%), followed by amikacin (64%). In contrast, trimethoprim–sulfamethoxazole and ceftazidime exhibited the lowest susceptibility rates (43% and 36%, respectively). High levels of resistance were observed to amoxicillin–clavulanic acid and vancomycin.
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